Journal: Cellular & molecular biology letters

Article Title: Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler.

doi: 10.1186/s11658-024-00573-x

Figure Lengend Snippet: Fig. 4 Characteristics of tissue layers of the antler growth center and isolated RM cells. A Immunofluorescence assay of CD73, CD90, Nestin, CD34, and Prrx1 (red color) in the RM, PC, and CA layers, respectively. B Third passage of cultured cells from the RM layer were identified using mesenchymal stem cell markers: CD73 and CD90 (red), Nestin and CD34 (green), respectively; nuclei were stained blue with DAPI. C Chondrogenic differentiation of RM cells, Alcian blue, and Col II immunofluorescence staining. D Osteogenic differentiation of RM cells with Alizarin red staining. E Adipogenic differentiation of RM cells with Oil Red O staining (scale bars, 125 μm and magnification ×200)

Article Snippet: The cells and tissues were fixed, permeabilized, blocked, and incubated overnight with primary antibodies: CD90 (1:300, proteintech, USA, 66766-1-lg), CD73 (1:1000, proteintech, USA, 12231- 1-AP), Nestin (1:300, BIOSS, China, bs-0008R), CD34 (1:300, proteintech, USA, 14486- 1-AP), and Prrx1 (1:200, Absin, China, abs134576).

Techniques: Isolation, Immunofluorescence, Cell Culture, Staining

Journal: International Journal of Molecular Sciences

Article Title: Induced Pluripotent Stem Cell-Derived Dendritic Cells Provide a Reliable In Vitro Platform for Functional Screening of Immunoregulatory Probiotics

doi: 10.3390/ijms27010303

Figure Lengend Snippet: Timeline and intermediate characterization of human-induced pluripotent stem cell (iPSC) differentiation into dendritic cells. ( a ) Timeline of human iPSC differentiation into dendritic cells. ( b ) Representative images of cellular morphology during differentiation at days 0, 12, 26, 31, and 32. Human iPSCs were cut into small pieces before seeding (Scale bar represents 200 μm). ( c ) Characterization of human iPSC-derived hematopoietic stem cells by flow cytometry. CD34 and CD45 were used as surface markers. ( d ) Characterization of human iPSC-derived monocytes by flow cytometry. CD14 was used as the surface marker.

Article Snippet: The antibodies used included: CD34-PE (Proteintech, Rosemont, IL, USA, #PE-65183), CD45-PE (BD Biosciences, Ashland, OR, USA, #555483), CD14-FITC (BD Biosciences, Ashland, OR, USA, #555397), CD11c-FITC (Proteintech, Rosemont, IL, USA, #FITC-65086), and CD83-PE (BD Biosciences, Ashland, OR, USA, #550634).

Techniques: Derivative Assay, Flow Cytometry, Marker